Initiation of embryogenic callus from mature zygotic embryos in Japanese larch (Larix kaempferi)
نویسندگان
چکیده
منابع مشابه
Pathogenicity of the pinewood nematode, Bursaphelenchus xylophilus, to Japanese larch, Larix kaempferi, seedlings.
Pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, to Japanese larch, Larix kaempferi, seedlings was tested with inoculation experiments under nursery conditions. Water suspensions of nematodes (mixed stages cultured on Botrytis cinerea or dispersal fourth-stage juveniles (DJ4) extracted from the adult Japanese pine sawyer, Monochamus alternatus) were injected into the stems o...
متن کاملGene expression associated with increased supercooling capability in xylem parenchyma cells of larch (Larix kaempferi).
Xylem parenchyma cells (XPCs) in larch adapt to subfreezing temperatures by deep supercooling, while cortical parenchyma cells (CPCs) undergo extracellular freezing. The temperature limits of supercooling in XPCs changed seasonally from -30 degrees C during summer to -60 degrees C during winter as measured by freezing resistance. Artificial deacclimation of larch twigs collected in winter reduc...
متن کاملEffects of exogenous mevalonic acid on sterol lipid classes in Larix kaempferi callus.
In Japanese larch (Larix kaempferi (Lamb.) Carr.) calli, free sterol (FS), acylsterol (AS) and glycosylsterol, including the acylated type, were found in the proportion of 1.0:0.1:0.8. When the calli were cultured in the presence of 10 mM mevalonic acid (MVA), the content of AS, but not FS and glycosylsterol, was increased remarkably. The major component sterol in each sterol lipid class was us...
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Organogenesis and neoformant plantlet production were studied by culturing the different parts of mature embryo including radicle and proximal and distal parts of cotyledon of Mission and Kroneiki cultivars. Different parts of embryos were cultures in OMc medium containing growth regulators, such as indole butyric acid (IBA) and 2-isopentenyl adenine (2-ip) or IBA and naphthalene acetic acid (N...
متن کاملNorway Spruce Somatic Embryogenesis: High Frequency Initiation from Light Cultured Mature Embryos Shirley A. Verhagen and Steven
Somatic embryos and rooted plantlets have been regenerated from light-initiated embryogenic callus derived from mature embryos of Picea abies stored seeds. Under a 16 hour photoperiod, mature zygotic embryos were cultured on a modified half-strength Murashige-Skoog medium without NH4NO3 and supplemented with 5 mM glutamine, 4.5 pM N6-benzyladenine (BA) and 10.7 PM naphthalene-acetic acid (NAA) ...
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ژورنال
عنوان ژورنال: Journal of Plant Biotechnology
سال: 2015
ISSN: 1229-2818,2384-1397
DOI: 10.5010/jpb.2015.42.3.223